il 10  (R&D Systems)

Journal: Cancer Biology & Medicine

Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis

doi: 10.20892/j.issn.2095-3941.2025.0282

Figure Lengend Snippet: MIIP suppresses M2 macrophage polarization and decreases macrophage-derived IL10 levels in a co-culture system. MIIP knockdown in SW480 cells increased CD163 expression (A) and CD206 levels (B) in PMA-induced THP-1 cells, the CD163 level in the co-culture medium (C), the mRNA expression of IL-10 (D), and the IL10 levels (E) in PMA-induced THP-1 cells. MIIP overexpression in CT26 cells decreased the CD163 level in the co-culture medium of the co-cultured system (F) and macrophage-derived IL10 expression (G) and IL-10 levels in RAW264.7 cells (H). Effects of MIIP knockdown, STING knockdown, and dual MIIP and STING knockdown in CRC cells on CD163 expression in co-cultured PMA-treated THP-1 cells (I), CD163 levels in the co-culture medium (J), CD206 levels in PMA-treated THP-1 cells (K), and macrophage-derived IL10 expression (L) and IL-10 levels (M). All data are presented as the means ± SDs ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; scale bar, 10 μm.

Article Snippet: NeutraKine ® IL-10 monoclonal antibody (10 ng/mL, 69018-1-Ig; Proteintech, Wuhan, Hubei, China), recombinant human IL-10 (10 ng/mL, HY- P70751 ; MedChemExpres, Monmouth Junction, NJ, USA), or recombinant mouse IL-10 (6 ng/mL, HY-P70517; MedChemExpres, Monmouth Junction, NJ, USA) was added to the lower chamber to assess the effect of IL-10.

Techniques: Derivative Assay, Co-Culture Assay, Knockdown, Expressing, Over Expression, Cell Culture

Journal: Cancer Biology & Medicine

Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis

doi: 10.20892/j.issn.2095-3941.2025.0282

Figure Lengend Snippet: MIIP inhibits tumor growth and metastasis and M2 macrophage infiltration in a syngeneic CRC mouse model through the STING–NFκB2–IL10 axis. (A) Tumors in situ (indicated by green arrows) and liver metastatic nodules (indicated by black arrows) were observed in a syngeneic CRC mouse model. Statistics for the size of tumors in situ (B) and the number of metastatic foci (C). (D) Statistical analysis of IL10 levels in mouse blood. (E) Representative images of HE and IHC staining for SATB2, MIIP, STING, IL10, p52, and CD163 (green arrows) in tumor tissues from the different groups. The bar graphs show the percentages of high/low STING (F), IL-10 (G), and p52 (H) expression in the different groups. (I) Comparison of the number of CD163-positive cells among the different groups. All data are presented as the means ± SDs ( n = 3). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; scale bar, 50 μm.

Article Snippet: NeutraKine ® IL-10 monoclonal antibody (10 ng/mL, 69018-1-Ig; Proteintech, Wuhan, Hubei, China), recombinant human IL-10 (10 ng/mL, HY- P70751 ; MedChemExpres, Monmouth Junction, NJ, USA), or recombinant mouse IL-10 (6 ng/mL, HY-P70517; MedChemExpres, Monmouth Junction, NJ, USA) was added to the lower chamber to assess the effect of IL-10.

Techniques: In Situ, Immunohistochemistry, Expressing, Comparison

Journal: Cancer Biology & Medicine

Article Title: Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING–NFκB2–IL10 axis

doi: 10.20892/j.issn.2095-3941.2025.0282

Figure Lengend Snippet: Schematic illustration of the mechanism by which MIIP haploinsufficiency promotes CRC progression through the modulation of CIN and M2 macrophage infiltration in the CRC microenvironment. In our previous study, MIIP haploinsufficiency was shown to induce excess APC/C Cdc20 ubiquitin ligase activity and the deregulation of Topo II activity, leading to CIN in CRC cells (in the red dotted line). The results of the current study further revealed that MIIP downregulation increases IL10 secretion via the dsDNA–STING–NFκB2–IL10 signaling axis in CRC cells and drives M2 macrophage polarization in the CRC tumor microenvironment. M2 macrophages promote CRC cell migration and invasion in turn in an IL-10-dependent manner (created with Figdraw.com ). APC/C, anaphase-promoting complex/cyclosome; MIIP, migration and invasion inhibitory protein; CIN, chromosomal instability; STING, stimulator of interferon genes; CRC, colorectal cancer.

Article Snippet: NeutraKine ® IL-10 monoclonal antibody (10 ng/mL, 69018-1-Ig; Proteintech, Wuhan, Hubei, China), recombinant human IL-10 (10 ng/mL, HY- P70751 ; MedChemExpres, Monmouth Junction, NJ, USA), or recombinant mouse IL-10 (6 ng/mL, HY-P70517; MedChemExpres, Monmouth Junction, NJ, USA) was added to the lower chamber to assess the effect of IL-10.

Techniques: Ubiquitin Proteomics, Activity Assay, Migration